Low Nutrient Experiment

In this experiment we are testing the effect of fertilizer on the speed of plant growth. We prepared a 4 quad cell, 1 control group and 3 experimental groups. So, we had one with no fertilizer, one with three seeds of fertilizer, one with six seeds of fertilizer, and lastly, one with nine seeds of fertilizer. The plants that we grew were called Wisconsin Fast Plants, members of the crucifer family. These plants are small and easy to grow, but for optimal growth they require continuous fertilizer, water, fluorescent light, and temperature between 18 degrees Celsius and 26 degrees Celsius 24 hours a day. Fertilizers are substances that are put into soils to increase the growth of the plant. There are two different types of fertilizers, synthetic

The growth and survival of a plant depends on the reactions that occur internally called photosynthesis. Photosynthesis is a reaction that captures the sun’s energy and converts it, water, and carbon dioxide into glucose, with a byproduct of oxygen. Glucose is a sugar that provides energy to allow for a plant to grow and live. This experiment is to test how photosynthesis can be sped up with a home solution. The variable being changed in this experiment is the solution that the plant is being given. In this instance, some of the plants will be given Gatorade, rather than water. The question being asked is, How well will a solution found in the home affect plant growth?

Background information: In this lab you will be looking at the growth of bacteria under different conditions to see the how populations of bacteria grow. Read about cells in the text or e-text. For everyone, in your e-text read chapter 13, sections 13.3 and 13.4 to learn more about bacteria. Then answer the questions below.

For an existing habitat, an increase in salt levels will begin to show an effect on the abundance and diversity in species of zooplankton. This will exhibit a detrimental effect on the daphnia from the limited osmoregulatory capabilities of the organisms. This in turn can pose a risk to the level feeding rates and survival (Heine-Fuster, Vega-Retter, Sabat & Ramos-Jiliberto,

As shown in the figure above, it is evident that V.natriegens grew faster when the Brain Heart Infusion (BHI) broth contained 250mM NaCl. The # of bacterial cells at each time point was measured following the equation given in the “How to generate a bacterial growth curve” supplemental material posted on D2L. (2) The data was then recorded in the table listed above. A growth curve graph was constructed using the data above which illustrated the differences between each of the different BHI mixtures. The graph was then used to determine the generation time of V.natriegens for each different environmental condition. In order to calculate the generation time (g) the mean growth rate (k) must be calculated. The formula to do this is posted in the supplemental material “How to generate a bacterial growth curve” on D2L. The k value calculated for each condition goes as follows:

[01.03]Sue conducts a series of experiments to investigate the effects of UV radiation on bacteria growth. In order to verify and strengthen her findings, she asks another scientist to conduct the same experiments in an independent investigation. The results of the second investigation are expected to be

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

This lab was conducted to see what happens when black tea and water are used to enhance the growth of a radish plant. The hypothesis was that the plants that were given the black tea and water mixture were going to grow faster than the control plant (Devonte). The hypothesis was partially correct because all the plants grew substantially large closer to the end of the lab. Plant 2 (Bravo) started to wither away and die because it was over fed, and eventually drowned in water. The other two plants grew at a consistent pace and developed into healthy mature plants. To sum up, the lab that was conducted was successful in the sense that two out of the four plants grew rapidly without the use of enhancements or

Bacteria grows by binary fission. The aim of this experiment is to follow the growth of Serratia marcescens in nutrient broth at 37oCby recording the changes in turbidity (cloudiness) by measuring the absorbance of visible light (600 nm) and also to prove that there is an increase in the cell number and not just in mass during the growth. In the experiment we measure the full growth curve of Serratia marcescens by measuring the absorbance at 600nm at every 10 mins. I also determined the viable count at the start and the end of the exponential phase of growth. Using the growth curve I calculated the growth curve and it was 1.2. Using this I found the doubling time which was 34s.

Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object

We can measure how certain variables like temperature, ph and oxygen levels affect on biological processes such a growth rate, reproduction and mortality. The organism we choose to study can be from different trophic levels to see how these changes affect the ecosystem as a whole and the food web. We can take measurements of these factors where they are high and see how they affect the organism. Additionally, we can test these variables individually in close systems to see what type of effects it has. In a video we watched in class we saw how rising pH could affect corals killing them off with coral bleaching and if acidic enough the coral itself would erode away because it was under the saturated state. Understanding these effects is essential to preserving our ocean and its

Alyssa E. Beck conducted an experiment in which her purpose was to observe the effects of short term ultraviolet light exposure on bacteria. In this experiment, she placed and labeled bacteria samples onto plates and exposed the bacteria to ultraviolet light for two, five, and thirty minutes. For each sample, half of the plate was exposed to the ultraviolet light while half was not, serving as the control group. The results of the experiment showed the bacteria not exposed to the uv light grew into distinct colonies while the none of the exposed bacteria survived after the time periods (Beck

In this lab experiment, students had to create a growth curve for E. coli. The E. coli growth curve would illustrate the progression of the population of E. coli a set time period. In this case, the growth curve depicted the population of E. coli over a 12-hour period. The growth curve for E. coli was created from the absorbance levels, the optical density(OD), recorded from the spectrophotometer.

Plankton are aquatic organisms that drift throughout their environment as they posses no true motor capabilities. Within their respective aquatic habitats plankton form the productive basis of their ecosystem and are divided into two subcategories: zooplankton and phytoplankton, the later will be the primary focus of this paper. Phytoplankton, as well as other photosynthetic organisms poses pigments called chlorophyll. These pigments allow phytoplankton to convert carbon dioxide and water to oxygen and sugar, which provides the phytoplankton with energy. Due to the fact that all phytoplankton posses chlorophyll scientists have developed methods that use chlorophyll testing in order to understand more about phytoplankton as a whole. Phytoplankton posses different types of chlorophyll but scientists usually sample chlorophyll-a as it is the most abundant form (YSI Environmental, Cullen 1982, Santos 2003). Several different methods have been developed to analyze the concentration of chlorophyll-a.

This experiment was performed to test the hypothesis if LB nutrient broth, +pGLO and -pGLO Ampicillin, and Arabinose was placed in the E. coli plates, then there will be a significant growth in the newly transformed bacteria and it will possess the ability to glow under UV light. The measurements were recorded from the bent glass tube in each glass test tube. The transformation protocol tested for the newly possessed traits in E.coli bacteria. Throughout the experiment there were many probable reasons for failure. If the pipettes and sterile loop were not thrown out in between each use, a cross contamination could cause a miscalculation in the experiment causing the data results to fail. The hypothesis that was tested was validated due to the positive results with each experiment stating that newly transformed organisms due in fact pass on traits.